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Registros recuperados : 104 | |
17. | | ANJOS, A. do; MAZZA, M. C. M.; SANTOS, A. C. M. C. dos; DELFINI, L. T. Anáhse do padrão de distribuição espacial da araucária (Araucaria angustifolia) em algumas áreas no Estado do Paraná, utilizrido a funcão K de Ripley Spatial point paern analysis of araucaria (Araucaria angustifolia) in some areas in the StaV ot Paraná, using Ripley?s K function Scientia Forestales, São Paulo, n.66, p.38-45, dez/2004. Biblioteca(s): Embrapa Rondônia. |
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20. | | MAZZA, C. A. da S.; MAZZA, M. C. M.; SANTOS, J. E. Análise ambiental da paisagem da Microrregião Colonial de Irati, PR. In: SANTOS, J. E. dos; CAVALHEIRO, F.; PIRES, J. S. R.; OLIVEIRA, C. H.; PIRES, A. M. Z. C. R. (Org.). Faces da polissemia da paisagem: ecologia, planejamento e percepção. São Carlos: RiMa, 2004. v. 2. p. 409-424. Biblioteca(s): Embrapa Florestas. |
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Registros recuperados : 104 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
31/03/1999 |
Data da última atualização: |
14/08/2012 |
Autoria: |
MAZZA, M. C. M. |
Afiliação: |
Pesquisadora da Embrapa-CNPF. |
Título: |
Use of rapd markers in the study of genetic diverstity of Araucaria angustifolia (Bert.) populations in Brazil. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
In: INTERNATIONAL FOUNDATION SCIENCE (Stockholm, Sweden). Recent advances in biotechnology for tree conservation and management: Proceedings of an INF Workshop, Florianopolis, Brazil, 15-19 september 1997. Stockholm: IFS / UFSC, 1997. |
Páginas: |
p. 103-111 |
ISBN: |
9185798-460 |
Idioma: |
Inglês |
Conteúdo: |
The araucaria forest (mixed ombrophilous forest with Araucaria angustifolia Bertoloni or the subtropical evergreen seasonal conifer forest) is one of the most important biomes that originally covered a large part of Southerm Brazil. With human population growth and expansion of agriculture, this forest was cut extensively and currently occupies less than 3% of its original area. large continuous forests wer also fragmented during this process and A. angustifolia populations are scattered in islands. This deforestation process probably ressulted in genetic erosion of A. angustifolia. Knowledge of diversity and distribution of genes within species and their local populations is an essential tool for the management and conservation of that species and biome. The aim of the present study was to analyse the genetic variability and differentiation among five populations of A. angustifolia through RAPD markers. Eleven DNA extraction protocols were tested in a factorial design, including use of different types of deproteinizing and antioxidant agents and detergents. Good quality DNA from buds of adult A. angustifolia trees was obtained only with a complex treatment which included addition of sarcosil to the extraction buffer, additional steps of phenol treatments, chloroform-isoamyl alcohol and low concentration-NaCl 1% CTAB reprecipitation. Other methods wer evalueted in attempts to simplify the process, increase yield and quality of DNA, and to decrease costs. The trial includes the determination of the leaf maturity stage from which good quality DNA could be obtained in large quantity. Quantity of DNA varied in the range of 20-1000 ng/ul, with higher values for buds and terminal needles. One hundred twenty random ten-base primers are being evaluated in five provenances, using bulking strategy, to determinate polymorphic and reproducible RAPD markers. Analysis of genetic structure in remnants of araucaria forests with the use of RAPD markers and gene flow between islands with the use of microsatellite markers will follow. MenosThe araucaria forest (mixed ombrophilous forest with Araucaria angustifolia Bertoloni or the subtropical evergreen seasonal conifer forest) is one of the most important biomes that originally covered a large part of Southerm Brazil. With human population growth and expansion of agriculture, this forest was cut extensively and currently occupies less than 3% of its original area. large continuous forests wer also fragmented during this process and A. angustifolia populations are scattered in islands. This deforestation process probably ressulted in genetic erosion of A. angustifolia. Knowledge of diversity and distribution of genes within species and their local populations is an essential tool for the management and conservation of that species and biome. The aim of the present study was to analyse the genetic variability and differentiation among five populations of A. angustifolia through RAPD markers. Eleven DNA extraction protocols were tested in a factorial design, including use of different types of deproteinizing and antioxidant agents and detergents. Good quality DNA from buds of adult A. angustifolia trees was obtained only with a complex treatment which included addition of sarcosil to the extraction buffer, additional steps of phenol treatments, chloroform-isoamyl alcohol and low concentration-NaCl 1% CTAB reprecipitation. Other methods wer evalueted in attempts to simplify the process, increase yield and quality of DNA, and to decrease costs. The trial includes t... Mostrar Tudo |
Palavras-Chave: |
Araucaraia angustifolia; Conservacao genetica; Estrutura genetica; Genetica de populacao; Genetics structure; Marcadores RAPD; Nature conservation. |
Thesaurus NAL: |
genetic markers; population genetics. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02998naa a2200253 a 4500 001 1303551 005 2012-08-14 008 1997 bl uuuu u00u1 u #d 020 $a9185798-460 100 1 $aMAZZA, M. C. M. 245 $aUse of rapd markers in the study of genetic diverstity of Araucaria angustifolia (Bert.) populations in Brazil. 260 $c1997 300 $ap. 103-111 520 $aThe araucaria forest (mixed ombrophilous forest with Araucaria angustifolia Bertoloni or the subtropical evergreen seasonal conifer forest) is one of the most important biomes that originally covered a large part of Southerm Brazil. With human population growth and expansion of agriculture, this forest was cut extensively and currently occupies less than 3% of its original area. large continuous forests wer also fragmented during this process and A. angustifolia populations are scattered in islands. This deforestation process probably ressulted in genetic erosion of A. angustifolia. Knowledge of diversity and distribution of genes within species and their local populations is an essential tool for the management and conservation of that species and biome. The aim of the present study was to analyse the genetic variability and differentiation among five populations of A. angustifolia through RAPD markers. Eleven DNA extraction protocols were tested in a factorial design, including use of different types of deproteinizing and antioxidant agents and detergents. Good quality DNA from buds of adult A. angustifolia trees was obtained only with a complex treatment which included addition of sarcosil to the extraction buffer, additional steps of phenol treatments, chloroform-isoamyl alcohol and low concentration-NaCl 1% CTAB reprecipitation. Other methods wer evalueted in attempts to simplify the process, increase yield and quality of DNA, and to decrease costs. The trial includes the determination of the leaf maturity stage from which good quality DNA could be obtained in large quantity. Quantity of DNA varied in the range of 20-1000 ng/ul, with higher values for buds and terminal needles. One hundred twenty random ten-base primers are being evaluated in five provenances, using bulking strategy, to determinate polymorphic and reproducible RAPD markers. Analysis of genetic structure in remnants of araucaria forests with the use of RAPD markers and gene flow between islands with the use of microsatellite markers will follow. 650 $agenetic markers 650 $apopulation genetics 653 $aAraucaraia angustifolia 653 $aConservacao genetica 653 $aEstrutura genetica 653 $aGenetica de populacao 653 $aGenetics structure 653 $aMarcadores RAPD 653 $aNature conservation 773 $tIn: INTERNATIONAL FOUNDATION SCIENCE (Stockholm, Sweden). Recent advances in biotechnology for tree conservation and management: Proceedings of an INF Workshop, Florianopolis, Brazil, 15-19 september 1997. Stockholm: IFS / UFSC, 1997.
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